Hot start reverse transcriptase: an approach for improved real-time RT-PCR performance
نویسندگان
چکیده
منابع مشابه
Reverse transcriptase-polymerase chain reaction (rt-PCR).
Reverse transcriptase polymerase chain reaction (RT-PCR) has become a well-established and powerful molecular technique for studying ribonucleic acids. It is used in medical diagnostics for detecting viral RNA, in hematology and oncology for detecting chimeric transcripts of rearranged genes (1), and in the broad area of research applications in gene expression studies. Since its introduction (...
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The polymerase chain reaction (PCR) is widely used for applications which require a high level of specificity and reliability, such as genetic testing, clinical diagnostics, blood screening, forensics and biodefense. Great improvements to PCR performance have been achieved by the use of Hot Start activation strategies that aim to prevent DNA polymerase extension until more stringent, higher tem...
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5 a Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary 6 Medicine, Iowa State University, Ames, Iowa, USA 7 b Laboratório de Pesquisa em Virologia Animal, Departamento de Medicina Veterinária 8 Preventiva, Escola de Veterinária, Universidade Federal de Minas Gerais, Brazil 9 Department of Biomedical Sciences and Pathobiology, Center for Molecular Medicine ...
متن کاملImproved PCR specificity with Hot Start PCR primers.
The polymerase chain reaction (PCR) is an indispensable DNA amplification technique that has been exploited in numerous areas, including molecular diagnostics. One major drawback of PCR is the competing amplification of undesired off-target products, which primarily occurs at ambient temperatures prior to PCR cycling (1). Hot Start PCR techniques aim to block the extension of primers in nonspec...
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Porcine respiratory disease complex (PRDC) continues to be a significant economic problem for swine producers. Porcine reproductive and respiratory syndrome virus (PRRSV), Swine influenza virus (SIV), Porcine circovirus-2 (PCV-2), Mycoplasma hyopneumoniae, Actinobacillus pleuropneumoniae, and Pasturella multocida are among the most important pathogens contributing to PRDC. Recently, a new gener...
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ژورنال
عنوان ژورنال: Journal of Analytical Science and Technology
سال: 2015
ISSN: 2093-3371
DOI: 10.1186/s40543-015-0063-4